Hotspot

trnF-GAA-ndhJ

LSC · 46724-47435 · 712 bp

Back to Suaeda

Pi 0.0881
MarkerSeek score 82.20
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
712
variable_sites
176
pi
0.0881
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGAAATACAAGATGCTTTTTGA 50.0 27.3 58.1 52.6 704-768 bp 0.8 44.0
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA TCCCAATTTTTATGAAATACAAGATGC 50.0 29.6 58.1 57.4 739-780 bp 0.8 44.0
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA CCCAATTTTTATGAAATACAAGATGCT 50.0 29.6 58.1 57.4 738-779 bp 0.8 44.0
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA ATGAAATACAAGATGCTTTTTGA 50.0 26.1 58.1 53.2 705-769 bp 0.8 44.0
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA CCCAATTTTTATGAAATACAAGATGC 50.0 30.8 58.1 56.2 738-779 bp 0.8 44.0