Hotspot

trnT-UGU-trnL-UAA

LSC · 45169-45761 · 593 bp

Back to Suaeda

Pi 0.0963
MarkerSeek score 84.40
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
593
variable_sites
168
pi
0.0963
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG AGCCAATTAAGTCCGTAGCG 55.0 50.0 57.9 58.1 666-765 bp 1.0 90.4
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT AGCCAATTAAGTCCGTAGCG 50.0 50.0 57.8 58.1 698-797 bp 1.0 89.6
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 654-753 bp 1.0 89.0
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG GCCAATTAAGTCCGTAGCGT 55.0 50.0 57.9 58.4 665-764 bp 1.0 88.1
trnT-UGU-trnL-UAA_p5 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 686-785 bp 1.0 88.1