Hotspot

trnS-GCU-trnG-UCC

LSC · 7429-8178 · 750 bp

Back to Suaeda

Pi 0.112
MarkerSeek score 83.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
750
variable_sites
231
pi
0.112
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CTTTCGTCCACTCAGCCATC ACGAATCACACTTTTACCACT 55.0 38.1 58.6 55.5 809-966 bp 1.0 58.6
trnS-GCU-trnG-UCC_p2 CTTTCGTCCACTCAGCCATC AGAACGAATCACACTTTTACCAC 55.0 39.1 58.6 57.3 812-969 bp 1.0 57.6
trnS-GCU-trnG-UCC_p3 CTTTCGTCCACTCAGCCATC GAACGAATCACACTTTTACCACT 55.0 39.1 58.6 57.3 811-968 bp 1.0 57.6
trnS-GCU-trnG-UCC_p4 CTTTCGTCCACTCAGCCATC AACGAATCACACTTTTACCACT 55.0 36.4 58.6 56.2 810-967 bp 1.0 56.7
trnS-GCU-trnG-UCC_p5 TTTCGTCCACTCAGCCATCT ACGAATCACACTTTTACCACT 50.0 38.1 59.0 55.5 808-965 bp 1.0 55.5