Hotspot

rps16-trnQ-UUG

LSC · 5559-6027 · 469 bp

Back to Suaeda

Pi 0.112
MarkerSeek score 81.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
469
variable_sites
142
pi
0.112
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 487-682 bp 1.0 90.3
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 498-693 bp 1.0 89.8
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TAAGGCATCGGGTTTTGGTC 45.0 50.0 58.1 58.2 572-712 bp 0.9 85.5
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC TAAGGCATCGGGTTTTGGTC 50.0 50.0 58.2 58.2 583-723 bp 0.9 85.0
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GTAAGGCATCGGGTTTTGGT 45.0 50.0 58.1 58.5 573-713 bp 0.9 83.2