primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	AGTTCTATATGTCTTTGGAATTCT	GGTAGAGTACTCGGCTTTTA	24	20	29.2	45.0	53.5	53.9	5.2	17.1	12.571138	3029	3762	751-784	1.000000	52.0
matK-trnK-UUU_p2	matK-trnK-UUU	2	AGTTCTATATGTCTTTGGAATTCTTC	GGTAGAGTACTCGGCTTTTA	26	20	30.8	45.0	55.4	53.9	5.2	17.1	12.703380	3029	3762	751-784	1.000000	52.0
matK-trnK-UUU_p3	matK-trnK-UUU	3	AGTTCTATATGTCTTTGGAATTCT	GGTAGAGTACTCGGCTTTTAA	24	21	29.2	42.9	53.5	54.8	5.2	17.1	12.708588	3029	3762	751-784	1.000000	52.0
matK-trnK-UUU_p4	matK-trnK-UUU	4	AGTTCTATATGTCTTTGGAATTCTT	GGTAGAGTACTCGGCTTTTA	25	20	28.0	45.0	54.3	53.9	5.2	17.1	12.806293	3029	3762	751-784	1.000000	52.0
matK-trnK-UUU_p5	matK-trnK-UUU	5	AGTTCTATATGTCTTTGGAATTCTTC	GGTAGAGTACTCGGCTTTTAA	26	21	30.8	42.9	55.4	54.8	5.2	17.1	12.840830	3029	3762	751-784	1.000000	52.0
rps16_p1	rps16	1	CCATTGAGTTAGCAACCCGA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.184051	4433	5558	2269-2454	0.900000	86.5
rps16_p2	rps16	2	CCATTGAGTTAGCAACCCGA	TGGGCTGAAATTGACGAACA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.133685	4433	5558	2383-2568	0.800000	82.9
rps16_p3	rps16	3	CCATTGAGTTAGCAACCCGA	ATAGCAATTTAGGTGGGGCG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.126693	4433	5558	2330-2515	0.700000	79.0
rps16_p4	rps16	4	ACCATTGAGTTAGCAACCCG	ATAGCAATTTAGGTGGGGCG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.197527	4433	5558	2331-2516	0.700000	78.4
rps16_p5	rps16	5	CCATTGAGTTAGCAACCCGA	GAATCCTTCCGTCCCAGAAC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.133539	4433	5558	2262-2367	0.500000	70.9
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5559	6027	487-682	1.000000	90.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5559	6027	498-693	1.000000	89.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.2	8.1	0.0	0.310345	5559	6027	572-712	0.900000	85.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	TAAGGCATCGGGTTTTGGTC	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.375253	5559	6027	583-723	0.900000	85.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	GTAAGGCATCGGGTTTTGGT	20	20	45.0	50.0	58.1	58.5	8.1	0.0	0.597945	5559	6027	573-713	0.900000	83.2
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CTTTCGTCCACTCAGCCATC	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.6	55.5	0.0	0.0	4.172099	7429	8178	809-966	1.000000	58.6
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	CTTTCGTCCACTCAGCCATC	AGAACGAATCACACTTTTACCAC	20	23	55.0	39.1	58.6	57.3	0.0	0.0	4.304815	7429	8178	812-969	1.000000	57.6
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CTTTCGTCCACTCAGCCATC	GAACGAATCACACTTTTACCACT	20	23	55.0	39.1	58.6	57.3	0.0	0.0	4.304815	7429	8178	811-968	1.000000	57.6
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CTTTCGTCCACTCAGCCATC	AACGAATCACACTTTTACCACT	20	22	55.0	36.4	58.6	56.2	0.0	0.0	4.412570	7429	8178	810-967	1.000000	56.7
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	TTTCGTCCACTCAGCCATCT	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	59.0	55.5	0.0	0.0	4.559202	7429	8178	808-965	1.000000	55.5
atpH-atpI_p1	atpH-atpI	1	GCCAGTGAACGAAGAAAACG	GGGGCACTTTTACCTTGGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.022654	12828	13561	1837-1879	0.900000	87.8
atpH-atpI_p2	atpH-atpI	2	GCCAGTGAACGAAGAAAACG	TTGGTCAGGGGCACTTTTAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.029011	12828	13561	1844-1886	0.900000	87.8
atpH-atpI_p3	atpH-atpI	3	ATAACGGAAGCGGCAGAAAT	GGGGCACTTTTACCTTGGAA	20	20	45.0	50.0	58.0	58.0	1.4	0.0	0.042324	12828	13561	1137-1178	0.900000	87.7
atpH-atpI_p4	atpH-atpI	4	CCAGTGAACGAAGAAAACGC	GGGGCACTTTTACCTTGGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.022654	12828	13561	1836-1878	0.800000	83.6
atpH-atpI_p5	atpH-atpI	5	CCAGTGAACGAAGAAAACGC	TTGGTCAGGGGCACTTTTAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.029011	12828	13561	1843-1885	0.800000	83.5
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	26864	27355	571-622	1.000000	88.5
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACAAT	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.343061	26864	27355	509-558	0.900000	85.3
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	26864	27355	518-569	1.000000	84.1
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	GACTACCATTAAAGCAGCCCA	20	21	50.0	47.6	58.1	58.0	0.0	0.0	1.082797	26864	27355	525-576	1.000000	83.3
trnC-GCA-petN_p5	trnC-GCA-petN	5	CCAGTTCAAATCCGGGTGTC	GCCCAAGCGAGACTTACAAT	20	20	55.0	50.0	58.8	58.3	0.0	0.0	1.095664	26864	27355	504-553	0.900000	79.2
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	CTAGCCTGCTCGTTTCAGTT	GTCTTCCCGAAATGGCTGAT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.130177	28166	28878	1404-1425	0.400000	62.8
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	TCGAGCATCTATCCACCAGT	GTCTTCCCGAAATGGCTGAT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.117375	28166	28878	1819-1886	0.300000	58.4
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TCGAGCATCTATCCACCAGT	TTCCCGAAATGGCTGATACG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.125148	28166	28878	1816-1883	0.300000	58.3
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	CTAGCCTGCTCGTTTCAGTT	TTCCCGAAATGGCTGATACG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.137950	28166	28878	1410-1422	0.300000	57.9
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	TCGAGCATCTATCCACCAGT	GATTCTTCCTGGGTTGGGTC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.203524	28166	28878	1958-1958	0.200000	46.4
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.196808	45169	45761	666-765	1.000000	90.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	AGCCAATTAAGTCCGTAGCG	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.304561	45169	45761	698-797	1.000000	89.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	45169	45761	654-753	1.000000	89.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.484236	45169	45761	665-764	1.000000	88.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	45169	45761	686-785	1.000000	88.1
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTTTTTGA	20	22	50.0	27.3	58.1	52.6	0.0	9.5	7.474357	46724	47435	704-768	0.800000	44.0
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	TCCCAATTTTTATGAAATACAAGATGC	20	27	50.0	29.6	58.1	57.4	0.0	0.0	7.650413	46724	47435	739-780	0.800000	44.0
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	CCCAATTTTTATGAAATACAAGATGCT	20	27	50.0	29.6	58.1	57.4	0.0	0.0	7.655817	46724	47435	738-779	0.800000	44.0
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	ATGAAATACAAGATGCTTTTTGA	20	23	50.0	26.1	58.1	53.2	0.0	9.5	7.831993	46724	47435	705-769	0.800000	44.0
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	CCCAATTTTTATGAAATACAAGATGC	20	26	50.0	30.8	58.1	56.2	0.0	0.0	7.848636	46724	47435	738-779	0.800000	44.0
petA-psbJ_p1	petA-psbJ	1	CAAGCCGTCCTTCCAGATAC	TGCGGGTTGGTTATTTGTCA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.076015	58820	59668	2295-2393	0.800000	82.4
petA-psbJ_p2	petA-psbJ	2	GGCGGCCGGTATAGTAAAAA	ACACAATCAAACCCGAACGA	20	20	50.0	45.0	58.0	58.0	29.4	0.0	0.091511	58820	59668	1567-1599	0.300000	55.3
petA-psbJ_p3	petA-psbJ	3	GGCGGCCGGTATAGTAAAAA	ACGACACAATCAAACCCGAA	20	20	50.0	45.0	58.0	58.0	29.4	0.0	0.091511	58820	59668	1570-1602	0.300000	55.3
petA-psbJ_p4	petA-psbJ	4	GGCGGCCGGTATAGTAAAAA	TGCGGGTTGGTTATTTGTCA	20	20	50.0	45.0	58.0	58.0	29.4	0.0	0.065147	58820	59668	1882-1914	0.300000	55.1
petA-psbJ_p5	petA-psbJ	5	GGCGGCCGGTATAGTAAAAA	GAGCCGACAAGGAATTCCAT	20	20	50.0	50.0	58.0	58.0	29.4	26.2	0.091930	58820	59668	1804-1836	0.300000	54.9
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AAATAACTAGTTAGAGTAGGCAT	20	23	45.0	30.4	58.0	52.4	0.0	19.6	8.610226	60492	61348	930-1278	1.000000	52.0
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AAATAACTAGTTAGAGTAGGCAT	20	23	50.0	30.4	57.9	52.4	6.4	19.6	8.644221	60492	61348	919-1267	1.000000	52.0
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	AAATAACTAGTTAGAGTAGGCAT	20	23	50.0	30.4	57.8	52.4	24.9	19.6	8.749230	60492	61348	842-1190	1.000000	52.0
psbE-petL_p4	psbE-petL	4	GTTCTCCTGTGCTTCCAGAC	AAATAACTAGTTAGAGTAGGCAT	20	23	55.0	30.4	58.2	52.4	0.9	19.6	8.790818	60492	61348	837-1185	1.000000	52.0
psbE-petL_p5	psbE-petL	5	AACCCGCAATGAATAGGGAC	AAATAACTAGTTAGAGTAGGCAT	20	23	50.0	30.4	57.9	52.4	6.4	19.6	8.645669	60492	61348	918-1009	0.900000	48.0
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	CACATTCTGGTGTCCTAGGC	AAACGGGTTTGGTGCTGTAA	20	20	55.0	45.0	58.0	58.2	3.0	0.0	0.264608	101727	103964	2923-2923	0.200000	46.0
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	GGTGACGATACTGTAGGGGA	AAACGGGTTTGGTGCTGTAA	20	20	55.0	45.0	57.9	58.2	0.0	0.0	0.297741	101727	103964	2852-2852	0.200000	45.7
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	ACGCCAGAAAGGTTCCTTTT	AAACGGGTTTGGTGCTGTAA	20	20	45.0	45.0	57.9	58.2	16.5	0.0	0.302136	101727	103964	2810-2810	0.200000	45.7
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	GAACTTGGGTGTGGGTCTTT	AAACGGGTTTGGTGCTGTAA	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.302136	101727	103964	2601-2601	0.200000	45.7
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	AATAGCTCGACGCCAGAAAG	AAACGGGTTTGGTGCTGTAA	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.306875	101727	103964	2819-2819	0.200000	45.6
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGCAGCGTTAAAAGCTTTT	CTCGCACTGCTTCCTAAGAG	20	20	45.0	55.0	57.9	58.1	26.5	0.0	0.228997	107226	107922	824-966	0.800000	82.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGCAGCGTTAAAAGCTTTT	CTCTCGCACTGCTTCCTAAG	20	20	45.0	55.0	57.9	58.1	26.5	0.0	0.228997	107226	107922	826-968	0.800000	82.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGCAGCGTTAAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.9	57.9	26.5	0.0	0.273193	107226	107922	813-955	0.800000	81.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGCAGCGTTAAAAGCTTTT	AACCATTGGGATTGGAGAGC	20	20	45.0	50.0	57.9	57.9	26.5	7.8	0.298889	107226	107922	1406-1538	0.800000	81.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGCAGCGTTAAAAGCTTTT	CCGACGTAGCAAATCCTTGA	20	20	45.0	50.0	57.9	58.0	26.5	0.0	0.153498	107226	107922	1359-1473	0.400000	66.7
ycf1_p1	ycf1	1	CTGGTGGCTTCTCTTCTTCC	GTGCCACGTTAGCCAGATTA	20	20	55.0	50.0	57.9	58.0	0.0	4.9	0.125019	122769	122888	1047-1095	0.200000	51.4
ycf1_p2	ycf1	2	CTGGTGGCTTCTCTTCTTCC	TTTTCCCAAGTGCCACGTTA	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.160709	122769	122888	1056-1104	0.200000	51.1
ycf1_p3	ycf1	3	GCTTAGCAACTGGTGGCTTA	GTGCCACGTTAGCCAGATTA	20	20	50.0	50.0	58.2	58.0	8.0	4.9	0.197325	122769	122888	1209-1257	0.200000	50.2
ycf1_p4	ycf1	4	CTGGTGGCTTCTCTTCTTCC	GAGCTACTGAGGAACAACGG	20	20	55.0	55.0	57.9	58.0	0.0	0.0	0.108808	122769	122888	1919-1967	0.200000	49.6
ycf1_p5	ycf1	5	TTACCGCCTTTTCGAATCGA	GAACAAGATAAGCCTGCGGA	20	20	45.0	50.0	57.6	58.0	0.0	0.0	0.391206	122163	122312	415-415	0.200000	45.1