Hotspot

ycf1

IRb · 113992-114348 · 357 bp

Back to Styphnolobium

Pi 0

MarkerSeek score 22.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 357
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	TACTTTGTTCGTTGGTGCCA	55.0	45.0	58.0	58.0	1431-1436 bp	1.0	80.2
ycf1_p2	CGGGAGAATTGATGACTGCA	TACTTTGTTCGTTGGTGCCA	50.0	45.0	58.0	58.0	1773-1778 bp	1.0	79.9
ycf1_p3	CAATTCCCGTTCTCAACCCA	TACTTTGTTCGTTGGTGCCA	50.0	45.0	58.1	58.0	1390-1395 bp	1.0	79.4
ycf1_p4	CGTTCTCAACCCATGACCAA	TACTTTGTTCGTTGGTGCCA	50.0	45.0	58.1	58.0	1383-1388 bp	1.0	79.3
ycf1_p5	GCCTCTCGGGAGAATTGATG	TACTTTGTTCGTTGGTGCCA	55.0	45.0	58.1	58.0	1779-1784 bp	1.0	79.1