Hotspot

rrn16S

IRa · 140822-142312 · 1491 bp

Back to Styphnolobium

Pi 0.000671

MarkerSeek score 30.40

Primer available yes

Label rrn16S

Feature Metadata

label_name: rrn16S
feature_type: rRNA
region: IRa
length_bp: 1491
variable_sites: 2
pi: 0.000671
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16S_p1	CAGACGTAGCTCGCTTCTTT	CAAGAAGAGGGGTTGATCCG	50.0	55.0	58.0	58.0	2241-2242 bp	1.0	80.1
rrn16S_p2	CAGACGTAGCTCGCTTCTTT	CCCGACTCCAAGTTGTTCAA	50.0	50.0	58.0	58.0	1953 bp	1.0	80.1
rrn16S_p3	AGGGGCTCCTATCAACTTGT	CAAGAAGAGGGGTTGATCCG	50.0	55.0	58.0	58.0	2345-2346 bp	1.0	80.0
rrn16S_p4	CAGACGTAGCTCGCTTCTTT	TCAGCTTTGTCCACGAACAA	50.0	45.0	58.0	58.0	1613 bp	0.75	69.6
rrn16S_p5	AGGGGCTCCTATCAACTTGT	TCAGCTTTGTCCACGAACAA	50.0	45.0	58.0	58.0	1717 bp	0.75	69.5