Hotspot

rpl16

LSC · 85859-87392 · 1534 bp

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Pi 0

MarkerSeek score 49.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1534
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AGACCTCTTTCCTTTGGTTCT	TCATTTGATATTGATCTAATTGCT	42.9	25.0	56.5	52.6	1582-1584 bp	1.0	40.1
rpl16_p2	AAGACCTCTTTCCTTTGGTTCT	TCATTTGATATTGATCTAATTGCT	40.9	25.0	57.2	52.6	1583-1585 bp	1.0	40.1
rpl16_p3	CCTAAAGACCTCTTTCCTTTGGT	TCATTTGATATTGATCTAATTGCT	43.5	25.0	58.0	52.6	1587-1589 bp	1.0	40.1
rpl16_p4	AGACCTCTTTCCTTTGGTTC	TCATTTGATATTGATCTAATTGCT	45.0	25.0	54.9	52.6	1582-1584 bp	1.0	40.1
rpl16_p5	GACCTCTTTCCTTTGGTTCT	TCATTTGATATTGATCTAATTGCT	45.0	25.0	54.9	52.6	1581-1583 bp	1.0	40.1