Hotspot

ndhA

SSC · 124047-126371 · 2325 bp

Back to Styphnolobium

Pi 0.00215

MarkerSeek score 44.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2325
variable_sites: 10
pi: 0.00215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGA	42.9	45.0	56.3	56.5	2447 bp	1.0	46.2
ndhA_p2	ACCCAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGAA	42.9	42.9	56.3	57.2	2447 bp	1.0	44.2
ndhA_p3	AACCCAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGA	40.9	45.0	57.0	56.5	2448 bp	1.0	44.2
ndhA_p4	TGAACCCAGTTACCATAAGGA	TGGGAGAAGTTGATCGTTGA	42.9	45.0	56.0	56.5	2450 bp	1.0	44.0
ndhA_p5	GAACCCAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGA	43.5	45.0	58.1	56.5	2449 bp	1.0	43.5