Hotspot

rps15-ycf1

SSC · 127941-128261 · 321 bp

Back to Styphnolobium

Pi 0

MarkerSeek score 49.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 321
variable_sites: 0
pi: 0
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CGATCCCGGGTTTTCTTCTT	ATGTGTCCGCGATTGAAAGT	50.0	45.0	57.9	58.2	407-411 bp	1.0	78.4
rps15-ycf1_p2	CGATCCCGGGTTTTCTTCTT	GTGTCCGCGATTGAAAGTTC	50.0	50.0	57.9	57.8	405-409 bp	1.0	78.1
rps15-ycf1_p3	CGATCCCGGGTTTTCTTCTT	TGTGTCCGCGATTGAAAGTT	50.0	45.0	57.9	58.4	406-410 bp	1.0	76.6
rps15-ycf1_p4	CGATCCCGGGTTTTCTTCTT	TCCGCGATTGAAAGTTCGTT	50.0	45.0	57.9	58.5	402-406 bp	1.0	76.0
rps15-ycf1_p5	CGATCCCGGGTTTTCTTCTT	ACATATGTGTCCGCGATTGA	50.0	45.0	57.9	57.4	411-415 bp	1.0	75.1