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psbE-petL

LSC · 68104-69476 · 1373 bp

Back to Styphnolobium

Pi 0.000364

MarkerSeek score 46.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1373
variable_sites: 1
pi: 0.000364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.0	58.0	1507-1529 bp	1.0	81.1
psbE-petL_p2	TGACAAATAACCAACCCGCA	AAGCCGCTAGTAGAAAACCG	45.0	50.0	58.0	58.0	1509-1531 bp	1.0	81.1
psbE-petL_p3	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.9	58.0	1496-1518 bp	1.0	80.9
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.9	58.0	1498-1520 bp	1.0	80.8
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	1419-1441 bp	1.0	80.1