Hotspot

atpB-rbcL

LSC · 57328-58080 · 753 bp

Back to Styphnolobium

Pi 0.000885
MarkerSeek score 50.10
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
753
variable_sites
1
pi
0.000885
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CCTGGGGGAAAAGCTACATC AACACCAGCTTTGAACCCAA 55.0 45.0 57.9 58.1 938 bp 1.0 78.6
atpB-rbcL_p2 CTGGGGGAAAAGCTACATCC AACACCAGCTTTGAACCCAA 55.0 45.0 57.9 58.1 937 bp 1.0 78.6
atpB-rbcL_p3 GGGGGAAAAGCTACATCCAG AACACCAGCTTTGAACCCAA 55.0 45.0 57.9 58.1 935 bp 1.0 78.6
atpB-rbcL_p4 AAGCTACATCCAGTACCGGA AACACCAGCTTTGAACCCAA 50.0 45.0 57.8 58.1 928 bp 1.0 77.8
atpB-rbcL_p5 ATTTGAGCGATACGTCCCAG AACACCAGCTTTGAACCCAA 50.0 45.0 57.8 58.1 902 bp 1.0 77.3