Hotspot

trnT-GGU-psbD

LSC · 34002-35486 · 1485 bp

Back to Styphnolobium

Pi 0.000337

MarkerSeek score 49.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1485
variable_sites: 1
pi: 0.000337
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CACAAAAACGAAACGGTCCC	55.0	50.0	58.1	58.2	1635-1636 bp	1.0	78.3
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	CCACAAAAACGAAACGGTCC	55.0	50.0	58.1	58.2	1636-1637 bp	1.0	78.3
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CCCACAAAAACGAAACGGTC	55.0	50.0	58.1	58.2	1637-1638 bp	1.0	78.3
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	CACAAAAACGAAACGGTCCC	50.0	50.0	58.1	58.2	1638-1639 bp	1.0	78.1
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CCACAAAAACGAAACGGTCC	50.0	50.0	58.1	58.2	1639-1640 bp	1.0	78.1