Hotspot

trnC-GCA-petN

LSC · 29283-30499 · 1217 bp

Back to Styphnolobium

Pi 0.00137

MarkerSeek score 52.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1217
variable_sites: 3
pi: 0.00137
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	57.9	58.0	1314-1336 bp	1.0	81.0
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	1258-1280 bp	1.0	80.9
trnC-GCA-petN_p3	TTTTGTATCGTTTTGGCGGC	CAGCCCAAGCAAGACTTACT	45.0	50.0	57.9	57.8	1308-1330 bp	1.0	79.6
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	1252-1274 bp	1.0	79.4
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	1276-1298 bp	1.0	78.7