Hotspot

trnS-GCU-trnT-CGU

LSC · 8546-9165 · 620 bp

Back to Styphnolobium

Pi 0.00161

MarkerSeek score 50.70

Primer available yes

Label trnS-GCU-trnT-CGU

Feature Metadata

label_name: trnS-GCU-trnT-CGU
feature_type: igs
region: LSC
length_bp: 620
variable_sites: 2
pi: 0.00161
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnT-CGU_p1	CAATCCGACGCTTTAGTCCA	GGGGTCCATAGAACGAATCG	50.0	55.0	58.0	57.8	703-713 bp	1.0	79.1
trnS-GCU-trnT-CGU_p2	AATCCGACGCTTTAGTCCAC	GGGGTCCATAGAACGAATCG	50.0	55.0	58.0	57.8	702-712 bp	1.0	79.1
trnS-GCU-trnT-CGU_p3	CAATCCGACGCTTTAGTCCA	GGTCCATAGAACGAATCGCA	50.0	50.0	58.0	57.8	701-711 bp	1.0	78.6
trnS-GCU-trnT-CGU_p4	AATCCGACGCTTTAGTCCAC	GGTCCATAGAACGAATCGCA	50.0	50.0	58.0	57.8	700-710 bp	1.0	78.5
trnS-GCU-trnT-CGU_p5	ACGGAAAGAGAGGGATTCGA	GGGGTCCATAGAACGAATCG	50.0	55.0	58.2	57.8	756-766 bp	1.0	77.9