Hotspot

trnK-UUU-rps16

LSC · 4465-5343 · 879 bp

Back to Styphnolobium

Pi 0.00284
MarkerSeek score 52.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
879
variable_sites
5
pi
0.00284
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCTACCGATGGTATGGACGA AACAAGGCGCTCAACCTAC 50.0 52.6 57.4 58.1 1065-1075 bp 1.0 67.5
trnK-UUU-rps16_p2 TCTACCGATGGTATGGACGA GAACAAGGCGCTCAACCTAC 50.0 55.0 57.4 59.2 1066-1076 bp 1.0 66.5
trnK-UUU-rps16_p3 TCTACCGATGGTATGGACGA GAACAAGGCGCTCAACCTA 50.0 52.6 57.4 57.8 1066-1076 bp 1.0 66.3
trnK-UUU-rps16_p4 CCGATGGTATGGACGAATCA AACAAGGCGCTCAACCTAC 50.0 52.6 57.2 58.1 1061-1071 bp 1.0 66.2
trnK-UUU-rps16_p5 ACCGATGGTATGGACGAATC AACAAGGCGCTCAACCTAC 50.0 52.6 57.2 58.1 1062-1072 bp 1.0 66.2