primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCTACCGATGGTATGGACGA	AACAAGGCGCTCAACCTAC	20	19	50.0	52.6	57.4	58.1	0.0	7.6	1.715579	4465	5343	1065-1075	1.000000	67.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TCTACCGATGGTATGGACGA	GAACAAGGCGCTCAACCTAC	20	20	50.0	55.0	57.4	59.2	0.0	7.6	1.847068	4465	5343	1066-1076	1.000000	66.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCTACCGATGGTATGGACGA	GAACAAGGCGCTCAACCTA	20	19	50.0	52.6	57.4	57.8	0.0	7.6	1.870879	4465	5343	1066-1076	1.000000	66.3
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGATGGTATGGACGAATCA	AACAAGGCGCTCAACCTAC	20	19	50.0	52.6	57.2	58.1	0.0	7.6	1.883942	4465	5343	1061-1071	1.000000	66.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	ACCGATGGTATGGACGAATC	AACAAGGCGCTCAACCTAC	20	19	50.0	52.6	57.2	58.1	0.0	7.6	1.885864	4465	5343	1062-1072	1.000000	66.2
trnS-GCU-trnT-CGU_p1	trnS-GCU-trnT-CGU	1	CAATCCGACGCTTTAGTCCA	GGGGTCCATAGAACGAATCG	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.154980	8546	9165	703-713	1.000000	79.1
trnS-GCU-trnT-CGU_p2	trnS-GCU-trnT-CGU	2	AATCCGACGCTTTAGTCCAC	GGGGTCCATAGAACGAATCG	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.156368	8546	9165	702-712	1.000000	79.1
trnS-GCU-trnT-CGU_p3	trnS-GCU-trnT-CGU	3	CAATCCGACGCTTTAGTCCA	GGTCCATAGAACGAATCGCA	20	20	50.0	50.0	58.0	57.8	0.0	1.2	0.223536	8546	9165	701-711	1.000000	78.6
trnS-GCU-trnT-CGU_p4	trnS-GCU-trnT-CGU	4	AATCCGACGCTTTAGTCCAC	GGTCCATAGAACGAATCGCA	20	20	50.0	50.0	58.0	57.8	0.0	1.2	0.224924	8546	9165	700-710	1.000000	78.5
trnS-GCU-trnT-CGU_p5	trnS-GCU-trnT-CGU	5	ACGGAAAGAGAGGGATTCGA	GGGGTCCATAGAACGAATCG	20	20	50.0	55.0	58.2	57.8	0.0	0.0	0.301746	8546	9165	756-766	1.000000	77.9
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	ACAAGCGGTATTCAAGCTCT	20	20	45.0	45.0	58.0	57.2	1.4	0.0	0.808551	14274	15647	1465-1485	1.000000	74.8
atpH-atpI_p2	atpH-atpI	2	GATCCCTTCTACAGCTTGCC	ACAAGCGGTATTCAAGCTCT	20	20	55.0	45.0	58.0	57.2	0.0	0.0	0.809747	14274	15647	1550-1570	1.000000	74.7
atpH-atpI_p3	atpH-atpI	3	AATAACGGAAGCGGCAGAAA	ACAAGCGGTATTCAAGCTCT	20	20	45.0	45.0	58.2	57.2	1.4	0.0	0.954089	14274	15647	1466-1486	1.000000	73.6
atpH-atpI_p4	atpH-atpI	4	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAAGGC	20	20	45.0	50.0	58.0	57.0	1.4	20.7	1.068024	14274	15647	1416-1436	1.000000	72.7
atpH-atpI_p5	atpH-atpI	5	GATCCCTTCTACAGCTTGCC	TAGGTGAATCCATGGAAGGC	20	20	55.0	50.0	58.0	57.0	0.0	20.7	1.069221	14274	15647	1501-1521	1.000000	72.7
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTTGTATCGTTTTGGCGGC	TTAAAGCAGCCCAAGCAAGA	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.059326	29283	30499	1314-1336	1.000000	81.0
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.088172	29283	30499	1258-1280	1.000000	80.9
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTTGTATCGTTTTGGCGGC	CAGCCCAAGCAAGACTTACT	20	20	45.0	50.0	57.9	57.8	0.0	0.0	0.244410	29283	30499	1308-1330	1.000000	79.6
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.273256	29283	30499	1252-1274	1.000000	79.4
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.4	58.0	14.7	0.0	0.361535	29283	30499	1276-1298	1.000000	78.7
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	CACAAAAACGAAACGGTCCC	20	20	55.0	50.0	58.1	58.2	7.6	0.0	0.233981	34002	35486	1635-1636	1.000000	78.3
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GTGGTAGAGTAACGCCATGG	CCACAAAAACGAAACGGTCC	20	20	55.0	50.0	58.1	58.2	7.6	0.0	0.233981	34002	35486	1636-1637	1.000000	78.3
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	CCCACAAAAACGAAACGGTC	20	20	55.0	50.0	58.1	58.2	7.6	0.0	0.233981	34002	35486	1637-1638	1.000000	78.3
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	CACAAAAACGAAACGGTCCC	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.256136	34002	35486	1638-1639	1.000000	78.1
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCAGTGGTAGAGTAACGCCA	CCACAAAAACGAAACGGTCC	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.256136	34002	35486	1639-1640	1.000000	78.1
atpB-rbcL_p1	atpB-rbcL	1	CCTGGGGGAAAAGCTACATC	AACACCAGCTTTGAACCCAA	20	20	55.0	45.0	57.9	58.1	0.0	0.0	0.199291	57328	58080	938-938	1.000000	78.6
atpB-rbcL_p2	atpB-rbcL	2	CTGGGGGAAAAGCTACATCC	AACACCAGCTTTGAACCCAA	20	20	55.0	45.0	57.9	58.1	4.2	0.0	0.199291	57328	58080	937-937	1.000000	78.6
atpB-rbcL_p3	atpB-rbcL	3	GGGGGAAAAGCTACATCCAG	AACACCAGCTTTGAACCCAA	20	20	55.0	45.0	57.9	58.1	12.8	0.0	0.199291	57328	58080	935-935	1.000000	78.6
atpB-rbcL_p4	atpB-rbcL	4	AAGCTACATCCAGTACCGGA	AACACCAGCTTTGAACCCAA	20	20	50.0	45.0	57.8	58.1	9.5	0.0	0.295265	57328	58080	928-928	1.000000	77.8
atpB-rbcL_p5	atpB-rbcL	5	ATTTGAGCGATACGTCCCAG	AACACCAGCTTTGAACCCAA	20	20	50.0	45.0	57.8	58.1	0.0	0.0	0.365766	57328	58080	902-902	1.000000	77.3
rbcL-accD_p1	rbcL-accD	1	AGTCCTGAATTAGCTGCTGC	CGGGTCTTCACTTACACTGG	20	20	50.0	55.0	58.0	57.9	13.4	0.0	0.115816	59530	60500	1155-1170	1.000000	80.3
rbcL-accD_p2	rbcL-accD	2	CTGCCAAATGGAGTCCTGAA	CGGGTCTTCACTTACACTGG	20	20	50.0	55.0	57.8	57.9	11.3	0.0	0.285308	59530	60500	1166-1181	1.000000	78.9
rbcL-accD_p3	rbcL-accD	3	GCTGCTGCTTGTGAAGTATG	CGGGTCTTCACTTACACTGG	20	20	50.0	55.0	57.5	57.9	8.5	0.0	0.619632	59530	60500	1143-1158	1.000000	76.3
rbcL-accD_p4	rbcL-accD	4	AGTCCTGAATTAGCTGCTGC	ACCCACGCCTGTATTCTAAC	20	20	50.0	50.0	58.0	57.3	13.4	0.0	0.721380	59530	60500	1096-1111	1.000000	75.5
rbcL-accD_p5	rbcL-accD	5	CTTGCTCGTGAGGGTAATGA	CGGGTCTTCACTTACACTGG	20	20	50.0	55.0	57.3	57.9	0.0	0.0	0.753247	59530	60500	1200-1215	1.000000	75.2
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	GCCGCTAGTAGAAAACCGAA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.017769	68104	69476	1507-1529	1.000000	81.1
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	AAGCCGCTAGTAGAAAACCG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.023310	68104	69476	1509-1531	1.000000	81.1
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.051764	68104	69476	1496-1518	1.000000	80.9
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.057305	68104	69476	1498-1520	1.000000	80.8
psbE-petL_p5	psbE-petL	5	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.156772	68104	69476	1419-1441	1.000000	80.1
trnP-GGG-psaJ_p1	trnP-GGG-psaJ	1	ACCCGTGACATTTTGTACCC	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.269767	70328	71070	838-861	1.000000	80.6
trnP-GGG-psaJ_p2	trnP-GGG-psaJ	2	CCCGTGACATTTTGTACCCA	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.271110	70328	71070	837-860	1.000000	80.6
trnP-GGG-psaJ_p3	trnP-GGG-psaJ	3	ACAGGATTTGAACCCGTGAC	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.277803	70328	71070	849-872	1.000000	80.5
trnP-GGG-psaJ_p4	trnP-GGG-psaJ	4	CAGGATTTGAACCCGTGACA	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.279136	70328	71070	848-871	1.000000	80.5
trnP-GGG-psaJ_p5	trnP-GGG-psaJ	5	TGACAGGATTTGAACCCGTG	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.279136	70328	71070	851-874	1.000000	80.5
clpP_p1	clpP	1	TATTTGCGCGGAATTCTTGC	ACCGATTGTTACGTTTCCACA	20	21	45.0	42.9	57.8	58.2	15.5	0.0	1.351089	74155	76182	2116-2118	1.000000	69.5
clpP_p2	clpP	2	CGTGTATTTGCGCGGAATTC	ACCGATTGTTACGTTTCCACA	20	21	50.0	42.9	58.5	58.2	15.5	0.0	1.711021	74155	76182	2120-2122	1.000000	66.6
clpP_p3	clpP	3	TATTTGCGCGGAATTCTTGC	CCGATTGTTACGTTTCCACA	20	20	45.0	45.0	57.8	56.4	15.5	0.0	1.803256	74155	76182	2115-2117	1.000000	65.8
clpP_p4	clpP	4	TATTTGCGCGGAATTCTTGC	ACCGATTGTTACGTTTCCAC	20	20	45.0	45.0	57.8	56.4	15.5	0.0	1.805661	74155	76182	2116-2118	1.000000	65.8
clpP_p5	clpP	5	ATCGTGTATTTGCGCGGAAT	ACCGATTGTTACGTTTCCACA	20	21	45.0	42.9	58.7	58.2	15.5	0.0	1.867946	74155	76182	2122-2124	1.000000	65.3
rps11-rpl36_p1	rps11-rpl36	1	ATTTTACGCGCATGCTTACG	CGGGGGCGAATTATAGTAAT	20	20	45.0	45.0	57.9	54.8	17.1	0.0	3.303687	83601	84112	643-643	1.000000	53.7
rps11-rpl36_p2	rps11-rpl36	2	ATTTTACGCGCATGCTTACG	CGGGGGCGAATTATAGTAATTTG	20	23	45.0	43.5	57.9	57.7	17.1	5.6	3.376826	83601	84112	643-643	1.000000	53.1
rps11-rpl36_p3	rps11-rpl36	3	TTGGTATTTTACGCGCATGC	CGGGGGCGAATTATAGTAAT	20	20	45.0	45.0	57.8	54.8	5.7	0.0	3.399580	83601	84112	648-648	1.000000	52.9
rps11-rpl36_p4	rps11-rpl36	4	CCCTTTGGTATTTTACGCGC	CGGGGGCGAATTATAGTAAT	20	20	50.0	45.0	57.8	54.8	5.7	0.0	3.401059	83601	84112	652-652	1.000000	52.9
rps11-rpl36_p5	rps11-rpl36	5	TTGGTATTTTACGCGCATGC	CGGGGGCGAATTATAGTAATTTG	20	23	45.0	43.5	57.8	57.7	5.7	5.6	3.472719	83601	84112	648-648	1.000000	52.3
rpl16_p1	rpl16	1	AGACCTCTTTCCTTTGGTTCT	TCATTTGATATTGATCTAATTGCT	21	24	42.9	25.0	56.5	52.6	0.0	0.0	11.912995	85859	87392	1582-1584	1.000000	40.1
rpl16_p2	rpl16	2	AAGACCTCTTTCCTTTGGTTCT	TCATTTGATATTGATCTAATTGCT	22	24	40.9	25.0	57.2	52.6	0.0	0.0	12.181317	85859	87392	1583-1585	1.000000	40.1
rpl16_p3	rpl16	3	CCTAAAGACCTCTTTCCTTTGGT	TCATTTGATATTGATCTAATTGCT	23	24	43.5	25.0	58.0	52.6	3.5	0.0	12.396623	85859	87392	1587-1589	1.000000	40.1
rpl16_p4	rpl16	4	AGACCTCTTTCCTTTGGTTC	TCATTTGATATTGATCTAATTGCT	20	24	45.0	25.0	54.9	52.6	0.0	0.0	12.511529	85859	87392	1582-1584	1.000000	40.1
rpl16_p5	rpl16	5	GACCTCTTTCCTTTGGTTCT	TCATTTGATATTGATCTAATTGCT	20	24	45.0	25.0	54.9	52.6	0.0	0.0	12.511529	85859	87392	1581-1583	1.000000	40.1
trnV-GAC-rrn16S_p1	trnV-GAC-rrn16S	1	AAGGGGTATAACTCAGCGGT	CCAGGATCGAACTCTCCATG	20	20	50.0	55.0	58.1	57.5	0.0	5.6	0.647348	105209	105430	318-318	1.000000	77.3
trnV-GAC-rrn16S_p2	trnV-GAC-rrn16S	2	TTGACGTGGTGGAAGTCATC	CCAGGATCGAACTCTCCATG	20	20	50.0	55.0	57.8	57.5	0.0	5.6	0.686654	105209	105430	287-287	1.000000	77.3
trnV-GAC-rrn16S_p3	trnV-GAC-rrn16S	3	CGTGGTGGAAGTCATCAGTT	CCAGGATCGAACTCTCCATG	20	20	50.0	55.0	57.8	57.5	0.0	5.6	0.692668	105209	105430	283-283	1.000000	77.3
trnV-GAC-rrn16S_p4	trnV-GAC-rrn16S	4	CTTGACGTGGTGGAAGTCAT	CCAGGATCGAACTCTCCATG	20	20	50.0	55.0	57.8	57.5	0.0	5.6	0.692668	105209	105430	288-288	1.000000	77.2
trnV-GAC-rrn16S_p5	trnV-GAC-rrn16S	5	TCTATTTCTCGATGGGGGCA	CCAGGATCGAACTCTCCATG	20	20	50.0	55.0	58.2	57.5	0.0	5.6	0.721593	105209	105430	342-342	1.000000	76.6
ycf1_p1	ycf1	1	GAGCTACTGAGGAACAACGG	TACTTTGTTCGTTGGTGCCA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.042149	113992	114348	1431-1436	1.000000	80.2
ycf1_p2	ycf1	2	CGGGAGAATTGATGACTGCA	TACTTTGTTCGTTGGTGCCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.068238	113992	114348	1773-1778	1.000000	79.9
ycf1_p3	ycf1	3	CAATTCCCGTTCTCAACCCA	TACTTTGTTCGTTGGTGCCA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.135531	113992	114348	1390-1395	1.000000	79.4
ycf1_p4	ycf1	4	CGTTCTCAACCCATGACCAA	TACTTTGTTCGTTGGTGCCA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.148942	113992	114348	1383-1388	1.000000	79.3
ycf1_p5	ycf1	5	GCCTCTCGGGAGAATTGATG	TACTTTGTTCGTTGGTGCCA	20	20	55.0	45.0	58.1	58.0	15.7	0.0	0.163496	113992	114348	1779-1784	1.000000	79.1
ndhA_p1	ndhA	1	ACCCAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGA	21	20	42.9	45.0	56.3	56.5	0.0	0.0	4.260965	124047	126371	2447-2447	1.000000	46.2
ndhA_p2	ndhA	2	ACCCAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGAA	21	21	42.9	42.9	56.3	57.2	0.0	0.0	4.510768	124047	126371	2447-2447	1.000000	44.2
ndhA_p3	ndhA	3	AACCCAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGA	22	20	40.9	45.0	57.0	56.5	0.0	0.0	4.517857	124047	126371	2448-2448	1.000000	44.2
ndhA_p4	ndhA	4	TGAACCCAGTTACCATAAGGA	TGGGAGAAGTTGATCGTTGA	21	20	42.9	45.0	56.0	56.5	0.0	0.0	4.545014	124047	126371	2450-2450	1.000000	44.0
ndhA_p5	ndhA	5	GAACCCAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGA	23	20	43.5	45.0	58.1	56.5	0.0	0.0	4.598821	124047	126371	2449-2449	1.000000	43.5
rps15-ycf1_p1	rps15-ycf1	1	CGATCCCGGGTTTTCTTCTT	ATGTGTCCGCGATTGAAAGT	20	20	50.0	45.0	57.9	58.2	22.9	8.2	0.301014	127941	128261	407-411	1.000000	78.4
rps15-ycf1_p2	rps15-ycf1	2	CGATCCCGGGTTTTCTTCTT	GTGTCCGCGATTGAAAGTTC	20	20	50.0	50.0	57.9	57.8	22.9	8.2	0.337178	127941	128261	405-409	1.000000	78.1
rps15-ycf1_p3	rps15-ycf1	3	CGATCCCGGGTTTTCTTCTT	TGTGTCCGCGATTGAAAGTT	20	20	50.0	45.0	57.9	58.4	22.9	8.2	0.520751	127941	128261	406-410	1.000000	76.6
rps15-ycf1_p4	rps15-ycf1	4	CGATCCCGGGTTTTCTTCTT	TCCGCGATTGAAAGTTCGTT	20	20	50.0	45.0	57.9	58.5	22.9	8.2	0.605066	127941	128261	402-406	1.000000	76.0
rps15-ycf1_p5	rps15-ycf1	5	CGATCCCGGGTTTTCTTCTT	ACATATGTGTCCGCGATTGA	20	20	50.0	45.0	57.9	57.4	22.9	8.2	0.704548	127941	128261	411-415	1.000000	75.1
rrn16S_p1	rrn16S	1	CAGACGTAGCTCGCTTCTTT	CAAGAAGAGGGGTTGATCCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.045866	140822	142312	2241-2242	1.000000	80.1
rrn16S_p2	rrn16S	2	CAGACGTAGCTCGCTTCTTT	CCCGACTCCAAGTTGTTCAA	20	20	50.0	50.0	58.0	58.0	0.0	1.4	0.054234	140822	142312	1953-1953	1.000000	80.1
rrn16S_p3	rrn16S	3	AGGGGCTCCTATCAACTTGT	CAAGAAGAGGGGTTGATCCG	20	20	50.0	55.0	58.0	58.0	0.9	0.0	0.057250	140822	142312	2345-2346	1.000000	80.0
rrn16S_p4	rrn16S	4	CAGACGTAGCTCGCTTCTTT	TCAGCTTTGTCCACGAACAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.047286	140822	142312	1613-1613	0.750000	69.6
rrn16S_p5	rrn16S	5	AGGGGCTCCTATCAACTTGT	TCAGCTTTGTCCACGAACAA	20	20	50.0	45.0	58.0	58.0	0.9	0.0	0.058671	140822	142312	1717-1717	0.750000	69.5