Hotspot

ycf1

SSC · 126158-131362 · 5205 bp

Back to Stylosanthes

Pi 0.0251

MarkerSeek score 56.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5205
variable_sites: 242
pi: 0.0251
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCAGTCAAGAAGGGGTCAT	AAATCGACGAACCCGAAGAG	50.0	50.0	58.0	58.0	470-476 bp	0.5	63.8
ycf1_p2	TCCAGTCAAGAAGGGGTCAT	CCTTTCCCTTGCCCTTTTCT	50.0	50.0	58.0	58.0	536-542 bp	0.5	63.8
ycf1_p3	TCCAGTCAAGAAGGGGTCAT	CGCTAGGTTTGAAAATGGCG	50.0	50.0	58.0	58.1	364-370 bp	0.5	63.8
ycf1_p4	GGCCATCGAGGAACTTCTTT	AAATCGACGAACCCGAAGAG	50.0	50.0	57.9	58.0	874 bp	0.25	49.0
ycf1_p5	GGCCATCGAGGAACTTCTTT	CCTTTCCCTTGCCCTTTTCT	50.0	50.0	57.9	58.0	940 bp	0.25	49.0