Hotspot

ndhF

SSC · 112167-114422 · 2256 bp

Back to Stylosanthes

Pi 0.0159

MarkerSeek score 54.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2256
variable_sites: 67
pi: 0.0159
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACCCCCTTGTCTTACCAAGT	GATCTCGAGGTGCGTTTCTT	50.0	50.0	58.2	58.0	2925-2942 bp	1.0	81.8
ndhF_p2	ACCCCCTTGTCTTACCAAGT	TGCTGTCCAAAAACCTCTCC	50.0	50.0	58.2	58.0	2977-2994 bp	1.0	81.6
ndhF_p3	ACCCCCTTGTCTTACCAAGT	AGGTGCGTTTCTTTGGAACT	50.0	45.0	58.2	57.9	2918-2935 bp	1.0	81.4
ndhF_p4	ACCCCCTTGTCTTACCAAGT	GGTGCGTTTCTTTGGAACTG	50.0	50.0	58.2	57.9	2917-2934 bp	1.0	80.9
ndhF_p5	ACCCCCTTGTCTTACCAAGT	TGGAACTGCCATGCAACTAA	50.0	45.0	58.2	57.7	2905-2922 bp	1.0	79.6