Hotspot

ndhF-rpl32

SSC · 114423-115041 · 619 bp

Back to Stylosanthes

Pi 0.0256

MarkerSeek score 67.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 619
variable_sites: 30
pi: 0.0256
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAAAGGT	GATCTCGAGGTGCGTTTCTT	45.0	50.0	55.2	58.0	686-703 bp	1.0	63.7
ndhF-rpl32_p2	CTGGAAGTGGAATGAAAGGT	AGGTGCGTTTCTTTGGAACT	45.0	45.0	55.2	57.9	679-696 bp	1.0	63.3
ndhF-rpl32_p3	CTGGAAGTGGAATGAAAGGT	GGTGCGTTTCTTTGGAACTG	45.0	50.0	55.2	57.9	678-695 bp	1.0	62.8
ndhF-rpl32_p4	CTGGAAGTGGAATGAAAGGT	GAGGTGCGTTTCTTTGGAAC	45.0	50.0	55.2	57.6	680-697 bp	1.0	60.5
ndhF-rpl32_p5	CTGGAAGTGGAATGAAAGGT	ATCTCGAGGTGCGTTTCTTT	45.0	45.0	55.2	57.5	685-702 bp	1.0	60.0