Hotspot

rpl20-rps12

LSC · 70122-70860 · 739 bp

Back to Stylosanthes

Pi 0.0199

MarkerSeek score 69.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 739
variable_sites: 27
pi: 0.0199
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CCCCCGAAAGCTTGATGTAA	ACAGCCAATCCGAAATGTCA	50.0	45.0	57.9	57.8	896-982 bp	1.0	79.8
rpl20-rps12_p2	CCCCCGAAAGCTTGATGTAA	ACAAGACAGCCAATCCGAAA	50.0	45.0	57.9	57.7	901-987 bp	1.0	79.2
rpl20-rps12_p3	TTCTCCTACGTTTCCCAGCT	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.4	57.8	864-950 bp	1.0	78.0
rpl20-rps12_p4	CCCCCGAAAGCTTGATGTAA	CACAAGACAGCCAATCCGAA	50.0	50.0	57.9	58.5	902-988 bp	1.0	77.5
rpl20-rps12_p5	CCCCCGAAAGCTTGATGTAA	AGACAGCCAATCCGAAATGT	50.0	45.0	57.9	57.5	898-984 bp	1.0	77.4