Hotspot

psbK-trnQ-UUG

LSC · 56557-57105 · 549 bp

Back to Stylosanthes

Pi 0.0273

MarkerSeek score 66.50

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 549
variable_sites: 27
pi: 0.0273
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	AGCAGCTTGCCAAACAAAAG	GTCTTTCAAGTCGCACGTTG	45.0	50.0	58.1	58.0	1244-1321 bp	1.0	86.5
psbK-trnQ-UUG_p2	CTTACAGCAGCTTGCCAAAC	GTCTTTCAAGTCGCACGTTG	50.0	50.0	57.9	58.0	1249-1326 bp	1.0	86.4
psbK-trnQ-UUG_p3	AGCAGCTTGCCAAACAAAAG	ATCAACTGTCCCCCAAACAC	45.0	50.0	58.1	58.0	1368-1445 bp	1.0	86.3
psbK-trnQ-UUG_p4	CTTACAGCAGCTTGCCAAAC	ATCAACTGTCCCCCAAACAC	50.0	50.0	57.9	58.0	1373-1450 bp	1.0	86.2
psbK-trnQ-UUG_p5	AGCAGCTTGCCAAACAAAAG	CATCAACTGTCCCCCAAACA	45.0	50.0	58.1	58.0	1369-1446 bp	1.0	86.2