Hotspot

trnfM-CAU-trnG-UCC

LSC · 25100-25241 · 142 bp

Back to Stylosanthes

Pi 0.0331

MarkerSeek score 71.90

Primer available yes

Label trnfM-CAU-trnG-UCC

Feature Metadata

label_name: trnfM-CAU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 9
pi: 0.0331
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnG-UCC_p1	CTTGAGGTCACGGGTTCAAA	TCTTTGCCAAGGAGAAGACG	50.0	50.0	58.0	57.8	220-246 bp	1.0	83.4
trnfM-CAU-trnG-UCC_p2	TGAGGTCACGGGTTCAAATC	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.8	57.8	218-244 bp	1.0	82.3
trnfM-CAU-trnG-UCC_p3	GTAGCTCGCAAGGCTCATAA	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.8	57.8	241-267 bp	1.0	81.5
trnfM-CAU-trnG-UCC_p4	TAGCTCGCAAGGCTCATAAC	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.8	57.8	240-266 bp	1.0	81.5
trnfM-CAU-trnG-UCC_p5	ATTCTTCTCTTTACCGCGGG	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.7	57.8	274-300 bp	1.0	80.3