Hotspot

trnF-GAA-trnL-UAA

LSC · 13618-13957 · 340 bp

Back to Stylosanthes

Pi 0.0291

MarkerSeek score 68.00

Primer available yes

Label trnF-GAA-trnL-UAA

Feature Metadata

label_name: trnF-GAA-trnL-UAA
feature_type: igs
region: LSC
length_bp: 340
variable_sites: 17
pi: 0.0291
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnL-UAA_p1	TCTACCAGCTGAGCTATCCC	AATCGTGAGGGTTCAAGTCC	55.0	50.0	58.0	57.5	394-408 bp	1.0	82.4
trnF-GAA-trnL-UAA_p2	TTTGAACTGGTGACACGAGG	AATCGTGAGGGTTCAAGTCC	50.0	50.0	58.1	57.5	430-444 bp	1.0	81.5
trnF-GAA-trnL-UAA_p3	TCAGTCCTCTGCTCTACCAG	AATCGTGAGGGTTCAAGTCC	55.0	50.0	57.9	57.5	406-420 bp	1.0	81.1
trnF-GAA-trnL-UAA_p4	GTGCCAGGAACCAGATTTGA	AATCGTGAGGGTTCAAGTCC	50.0	50.0	58.1	57.5	445-459 bp	1.0	81.0
trnF-GAA-trnL-UAA_p5	TCTACCAGCTGAGCTATCCC	GGAAGAGGAAAATCCGTCGA	55.0	50.0	58.0	57.3	421-435 bp	1.0	80.4