Hotspot

ndhJ-trnF-GAA

LSC · 12722-13544 · 823 bp

Back to Stylosanthes

Pi 0.024

MarkerSeek score 67.10

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 37
pi: 0.024
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	TTGGATAGGATGGCCTTTGC	GGGATAGCTCAGCTGGTAGA	50.0	55.0	57.9	58.0	963-971 bp	1.0	83.1
ndhJ-trnF-GAA_p2	TTGGATAGGATGGCCTTTGC	CCTCGTGTCACCAGTTCAAA	50.0	50.0	57.9	58.1	927-935 bp	1.0	82.9
ndhJ-trnF-GAA_p3	TTGGATAGGATGGCCTTTGC	CTGGTAGAGCAGAGGACTGA	50.0	55.0	57.9	57.9	951-959 bp	1.0	82.1
ndhJ-trnF-GAA_p4	ATGCCCGAAAGTTGGATAGG	GGGATAGCTCAGCTGGTAGA	50.0	55.0	57.7	58.0	974-982 bp	1.0	80.9
ndhJ-trnF-GAA_p5	ATGCCCGAAAGTTGGATAGG	CCTCGTGTCACCAGTTCAAA	50.0	50.0	57.7	58.1	938-946 bp	1.0	80.6