Hotspot

atpE-trnM-CAU

LSC · 9290-9427 · 138 bp

Back to Stylosanthes

Pi 0.0426

MarkerSeek score 67.90

Primer available yes

Label atpE-trnM-CAU

Feature Metadata

label_name: atpE-trnM-CAU
feature_type: igs
region: LSC
length_bp: 138
variable_sites: 11
pi: 0.0426
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE-trnM-CAU_p1	CGAGCTAGGACACGAGTAGA	TACTGGACGTCTCAACCCTT	55.0	50.0	58.1	58.0	620-632 bp	0.75	73.3
atpE-trnM-CAU_p2	AGCTGAAGGCAAGAGACAAC	TACTGGACGTCTCAACCCTT	50.0	50.0	58.1	58.0	666-678 bp	0.75	72.7
atpE-trnM-CAU_p3	GCTGAAGGCAAGAGACAACT	TACTGGACGTCTCAACCCTT	50.0	50.0	58.1	58.0	665-677 bp	0.75	72.7
atpE-trnM-CAU_p4	CTCTGATGGGTGGTTTTGCT	TACTGGACGTCTCAACCCTT	50.0	50.0	58.1	58.0	799-811 bp	0.75	72.4
atpE-trnM-CAU_p5	TGCCGAGAAGAGTAGTGACA	TACTGGACGTCTCAACCCTT	50.0	50.0	58.1	58.0	741-753 bp	0.75	72.4