Hotspot

trnK-UUU-rbcL

LSC · 4400-5142 · 743 bp

Back to Stylosanthes

Pi 0.029

MarkerSeek score 64.90

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 39
pi: 0.029
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	CCTTTCTGGATCAGTCGTGG	AATGAGGGTCGTGATCTTGC	55.0	50.0	58.0	58.0	1006-1025 bp	1.0	83.9
trnK-UUU-rbcL_p2	CCTTTCTGGATCAGTCGTGG	AGTCCTGAATTAGCTGCTGC	55.0	50.0	58.0	58.0	946-965 bp	1.0	83.9
trnK-UUU-rbcL_p3	CATTGATCGCCACCCCATAA	AATGAGGGTCGTGATCTTGC	50.0	50.0	58.0	58.0	1348-1371 bp	1.0	83.0
trnK-UUU-rbcL_p4	TTGATCGCCACCCCATAATG	AATGAGGGTCGTGATCTTGC	50.0	50.0	58.0	58.0	1346-1369 bp	1.0	83.0
trnK-UUU-rbcL_p5	TTGATCGCCACCCCATAATG	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.0	58.0	1286-1309 bp	1.0	82.9