Hotspot

trnL-UAG-rpl32

SSC · 121175-121738 · 564 bp

Back to Stylidium

Pi 0.0525

MarkerSeek score 75.30

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 564
variable_sites: 29
pi: 0.0525
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	GGCGGCATTAAAAGCTTTGT	50.0	45.0	58.0	57.9	681 bp	1.0	84.5
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	GGCGGCATTAAAAGCTTTGT	50.0	45.0	58.0	57.9	682 bp	1.0	84.5
trnL-UAG-rpl32_p3	AATAAACCCAACGGCCACTT	GGCGGCATTAAAAGCTTTGT	45.0	45.0	58.0	57.9	1045-1046 bp	1.0	82.9
trnL-UAG-rpl32_p4	AATAAACCCAACGGCCACTT	GGATACTCGGCGGCATTAAA	45.0	50.0	58.0	58.1	1053-1054 bp	1.0	82.5
trnL-UAG-rpl32_p5	AGCTGGTACCAATATTGCGG	GGCGGCATTAAAAGCTTTGT	50.0	45.0	58.0	57.9	1248 bp	0.5	59.1