Hotspot

ndhA-ndhI

SSC · 115760-115845 · 86 bp

Back to Stylidium

Pi 0.0476

MarkerSeek score 78.80

Primer available yes

Label ndhA-ndhI

Feature Metadata

label_name: ndhA-ndhI
feature_type: igs
region: SSC
length_bp: 86
variable_sites: 4
pi: 0.0476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA-ndhI_p1	ACCAAGACTTAGAATGGATCAACT	TGACCAATATACCTTGCGGC	37.5	50.0	57.6	58.0	264-266 bp	1.0	46.5
ndhA-ndhI_p2	ACCAAGACTTAGAATGGATCAACT	CAATATACCTTGCGGCTCGT	37.5	50.0	57.6	58.1	260-262 bp	1.0	45.7
ndhA-ndhI_p3	ACCAAGACTTAGAATGGATCAACT	ACCAATATACCTTGCGGCTC	37.5	50.0	57.6	57.7	262-264 bp	1.0	44.7
ndhA-ndhI_p4	ACCAAGACTTAGAATGGATCAACT	GACCAATATACCTTGCGGCT	37.5	50.0	57.6	57.7	263-265 bp	1.0	44.7
ndhA-ndhI_p5	ACCAAGACTTAGAATGGATCAACT	GCTCGTACTGTCTGTTGACC	37.5	55.0	57.6	58.3	246-248 bp	1.0	44.5