Hotspot

petL-petG

LSC · 64060-64241 · 182 bp

Back to Stylidium

Pi 0.0686
MarkerSeek score 73.70
Primer available yes
Label petL-petG

Feature Metadata

label_name
petL-petG
feature_type
igs
region
LSC
length_bp
182
variable_sites
12
pi
0.0686
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petL-petG_p1 TGAGCAAGATACGACTTATTTGA GCCGCGCCTGTATTGTAAAT 34.8 50.0 55.8 59.3 296-301 bp 1.0 45.1
petL-petG_p2 TGAGCAAGATACGACTTATTTG GCCGCGCCTGTATTGTAAAT 36.4 50.0 54.3 59.3 296-301 bp 1.0 45.1
petL-petG_p3 TGAGCAAGATACGACTTATTTGA GCCGCGCCTGTATTGTAAA 34.8 52.6 55.8 58.9 296-301 bp 1.0 45.1
petL-petG_p4 TGAGCAAGATACGACTTATTTGA CCGCGCCTGTATTGTAAATA 34.8 45.0 55.8 56.0 295-300 bp 1.0 45.1
petL-petG_p5 GAGCAAGATACGACTTATTTGA GCCGCGCCTGTATTGTAAAT 36.4 50.0 54.1 59.3 295-300 bp 1.0 45.1