Hotspot

accD-psaI

LSC · 57031-57777 · 747 bp

Back to Stylidium

Pi 0.0376

MarkerSeek score 69.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 27
pi: 0.0376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGTGACAGCGAGTTTTGGTA	TGATTGCCGGAAATACGAGG	50.0	50.0	58.1	58.1	1044-1064 bp	1.0	83.1
accD-psaI_p2	GGTGACAGCGAGTTTTGGTA	TTGCTCGGACGGAAATAAGG	50.0	50.0	58.1	58.0	1586-1644 bp	1.0	82.6
accD-psaI_p3	ATGGGATCCGTAGTTGGTGA	TTGCTCGGACGGAAATAAGG	50.0	50.0	58.1	58.0	1804 bp	0.5	58.8
accD-psaI_p4	ATGGGATCCGTAGTTGGTGA	TGATTGCCGGAAATACGAGG	50.0	50.0	58.1	58.1	1282 bp	0.5	58.6
accD-psaI_p5	ATGGGATCCGTAGTTGGTGA	AAAAAGACCCGCAATCCCAT	50.0	45.0	58.1	58.1	1861 bp	0.5	58.5