Hotspot

trnS-GGA-rps4

LSC · 43764-43943 · 180 bp

Back to Stylidium

Pi 0.0944
MarkerSeek score 80.40
Primer available yes
Label trnS-GGA-rps4

Feature Metadata

label_name
trnS-GGA-rps4
feature_type
igs
region
LSC
length_bp
180
variable_sites
17
pi
0.0944
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-rps4_p1 AGGCTTTTGTTTACCGAGGG ATGAATTGCTGGTGGTAGAA 50.0 40.0 58.1 54.6 261-264 bp 1.0 57.4
trnS-GGA-rps4_p2 AGGCTTTTGTTTACCGAGGG TGAATTGCTGGTGGTAGAAT 50.0 40.0 58.1 54.6 260-263 bp 1.0 57.4
trnS-GGA-rps4_p3 GTAGGAAAGATGGCCGAGTG AATGAATTGCTGGTGGTAGA 55.0 40.0 58.1 54.6 312-315 bp 1.0 56.8
trnS-GGA-rps4_p4 GTAGGAAAGATGGCCGAGTG ATGAATTGCTGGTGGTAGAA 55.0 40.0 58.1 54.6 311-314 bp 1.0 56.8
trnS-GGA-rps4_p5 GTAGGAAAGATGGCCGAGTG TGAATTGCTGGTGGTAGAAT 55.0 40.0 58.1 54.6 310-313 bp 1.0 56.8