Hotspot

trnC-GCA-petN

LSC · 27205-27992 · 788 bp

Back to Stylidium

Pi 0.0374

MarkerSeek score 74.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 28
pi: 0.0374
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCGAGATTTACT	50.0	50.0	58.1	58.0	811-845 bp	1.0	85.6
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCGAGATTTACT	50.0	50.0	58.4	58.0	829-863 bp	1.0	83.3
trnC-GCA-petN_p3	TTCCCTATTGTGTACCGTGC	CAGCCCAAGCGAGATTTACT	50.0	50.0	57.6	58.0	874-908 bp	1.0	82.7
trnC-GCA-petN_p4	CCAGTTCAAATCCGGGTGTC	CAGCCCAAGCGAGATTTACT	55.0	50.0	58.8	58.0	806-840 bp	1.0	79.6
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	818-852 bp	1.0	78.5