Hotspot

atpF-atpH

LSC · 12197-12483 · 287 bp

Back to Stylidium

Pi 0.0507

MarkerSeek score 79.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 287
variable_sites: 14
pi: 0.0507
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCCCAAAGAAACGAAAGA	TTATGGCCTGGTTGTAGCAC	45.0	50.0	57.9	57.9	367-377 bp	1.0	83.9
atpF-atpH_p2	GTGGCCCAAAGAAACGAAAG	TTATGGCCTGGTTGTAGCAC	50.0	50.0	57.9	57.9	368-378 bp	1.0	83.3
atpF-atpH_p3	TGGCCCAAAGAAACGAAAGA	CCTGGTTGTAGCACTAGCAC	45.0	55.0	57.9	58.3	361-371 bp	1.0	82.8
atpF-atpH_p4	AGTGGCCCAAAGAAACGAAA	TTATGGCCTGGTTGTAGCAC	45.0	50.0	58.2	57.9	369-379 bp	1.0	82.5
atpF-atpH_p5	GTGGCCCAAAGAAACGAAAG	CCTGGTTGTAGCACTAGCAC	50.0	55.0	57.9	58.3	362-372 bp	1.0	82.2