Hotspot

rps16-trnQ-UUG

LSC · 5840-6192 · 353 bp

Back to Stylidium

Pi 0.0562

MarkerSeek score 75.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 353
variable_sites: 19
pi: 0.0562
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	433-539 bp	1.0	84.9
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	426-532 bp	1.0	84.0
rps16-trnQ-UUG_p3	CCACACCGGATCATGTCTTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	447-553 bp	1.0	83.9
rps16-trnQ-UUG_p4	ATGTCTTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	435-541 bp	1.0	83.9
rps16-trnQ-UUG_p5	CATGTCTTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	436-542 bp	1.0	83.2