Hotspot

matK-trnK-UUU

LSC · 3148-3880 · 733 bp

Back to Stylidium

Pi 0.0447
MarkerSeek score 72.50
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
733
variable_sites
32
pi
0.0447
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 AGTAGCGAAGAACTTGCACC AACGGTAGAGTACTCGGCTT 50.0 50.0 58.2 58.2 1244 bp 0.5 57.0
matK-trnK-UUU_p2 AGTAGCGAAGAACTTGCACC ACGGTAGAGTACTCGGCTTT 50.0 50.0 58.2 58.2 1243 bp 0.5 57.0
matK-trnK-UUU_p3 TAGCGAAGAACTTGCACCAA AACGGTAGAGTACTCGGCTT 45.0 50.0 57.8 58.2 1242 bp 0.5 56.7
matK-trnK-UUU_p4 TAGCGAAGAACTTGCACCAA ACGGTAGAGTACTCGGCTTT 45.0 50.0 57.8 58.2 1241 bp 0.5 56.7
matK-trnK-UUU_p5 AGTAGCGAAGAACTTGCACC ATTTGAAGATGCGTGTGTGC 50.0 45.0 58.2 57.7 1453 bp 0.5 55.7