Hotspot

ycf1

IRb · 111100-116517 · 5418 bp

Back to Strumaria

Pi 0.115

MarkerSeek score 68.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 5418
variable_sites: 1147
pi: 0.115
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.63
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAGGAACAAGAGGGATCCA	CGCGTTTGCCTTTTCTTGAA	50.0	45.0	58.0	58.2	1037-1049 bp	0.285714	50.6
ycf1_p2	ACAGGAACAAGAGGGATCCA	ATTCTCCGCCAAAGCTAACC	50.0	50.0	58.0	58.2	752 bp	0.285714	49.7
ycf1_p3	ACAGGAACAAGAGGGATCCA	TGCGCGCGTTTAAAAGAAAA	50.0	40.0	58.0	58.2	804 bp	0.142857	43.9
ycf1_p4	ACAGGAACAAGAGGGATCCA	TTGCGCGCGTTTAAAAGAAA	50.0	40.0	58.0	58.2	805 bp	0.142857	43.9
ycf1_p5	ACAGGAACAAGAGGGATCCA	CTGAATCAATTCCTTGCGCG	50.0	50.0	58.0	58.2	818 bp	0.142857	43.9