Hotspot

ycf1-rpl32

SSC · 116518-117226 · 709 bp

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Pi 0.0414

MarkerSeek score 72.20

Primer available yes

Label ycf1-rpl32

Feature Metadata

label_name: ycf1-rpl32
feature_type: igs
region: SSC
length_bp: 709
variable_sites: 62
pi: 0.0414
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rpl32_p1	ATCTCCCAGACGTCGTAGAG	AATGCTTAGAACCGGCAGAC	55.0	50.0	58.0	58.3	1333-2423 bp	0.285714	50.4
ycf1-rpl32_p2	ATGGAATGCCCCCTTGAATC	AATGCTTAGAACCGGCAGAC	50.0	50.0	57.9	58.3	1744-2834 bp	0.285714	50.0
ycf1-rpl32_p3	GATGGAATGCCCCCTTGAAT	AATGCTTAGAACCGGCAGAC	50.0	50.0	57.9	58.3	1745-2835 bp	0.285714	50.0
ycf1-rpl32_p4	GGAATGCCCCCTTGAATCAA	AATGCTTAGAACCGGCAGAC	50.0	50.0	58.1	58.3	1742-2832 bp	0.285714	49.6
ycf1-rpl32_p5	ACCAATAACGGCGGTCATTT	AATGCTTAGAACCGGCAGAC	45.0	50.0	58.2	58.3	1095-2185 bp	0.285714	49.5