Hotspot

petA-psbJ

LSC · 64452-65570 · 1119 bp

Back to Strumaria

Pi 0.0198

MarkerSeek score 71.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1119
variable_sites: 51
pi: 0.0198
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTTT	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.4	58.3	1261-1283 bp	1.0	76.8
petA-psbJ_p2	CGTCCAAGGCCTTTTGTTTT	CATATTCCGGGTTGGGTTCA	45.0	50.0	57.4	57.6	1257-1279 bp	1.0	75.8
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTTT	GTTCATATTCCGGGTTGGGT	45.0	50.0	57.4	57.6	1260-1282 bp	1.0	75.8
petA-psbJ_p4	GATCCATTACGCGTCCAAGG	GGTTCATATTCCGGGTTGGG	55.0	55.0	58.8	58.3	1272-1294 bp	1.0	75.2
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTTT	CGGTATTCCTGTGATCGGTT	45.0	50.0	57.4	57.4	1301-1323 bp	1.0	74.4