Hotspot

rbcL-accD

LSC · 58028-58817 · 790 bp

Back to Strumaria

Pi 0.0174

MarkerSeek score 65.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 790
variable_sites: 31
pi: 0.0174
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGCTGCTTGTGAAGTATGGA	CAACATCGAATTGAACCGCC	50.0	50.0	58.3	58.1	858-922 bp	1.0	83.8
rbcL-accD_p2	CGCTGCTTGTGAAGTATGGA	ACATCGAATTGAACCGCCAT	50.0	45.0	58.3	58.2	856-920 bp	1.0	82.6
rbcL-accD_p3	CGCTGCTTGTGAAGTATGGA	AACATCGAATTGAACCGCCA	50.0	45.0	58.3	58.5	857-921 bp	1.0	80.8
rbcL-accD_p4	CGCTGCTTGTGAAGTATGGA	ACAACATCGAATTGAACCGC	50.0	45.0	58.3	57.4	859-923 bp	1.0	79.7
rbcL-accD_p5	CGCTGCTTGTGAAGTATGGA	CATCGAATTGAACCGCCATT	50.0	45.0	58.3	57.2	855-919 bp	1.0	77.8