Hotspot

atpB-rbcL

LSC · 55712-56587 · 876 bp

Back to Strumaria

Pi 0.026

MarkerSeek score 74.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 876
variable_sites: 50
pi: 0.026
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TCTTCAAGTGTGGAAACCGC	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.7	56.6	907-990 bp	1.0	71.6
atpB-rbcL_p2	CGCAGGACCAGAAGTAGTAG	AGTCTCTGTTTGTGGTGACA	55.0	45.0	57.1	56.6	890-973 bp	1.0	70.4
atpB-rbcL_p3	CTTCAAGTGTGGAAACCGCA	AGTCTCTGTTTGTGGTGACA	50.0	45.0	59.0	56.6	906-989 bp	1.0	69.4
atpB-rbcL_p4	TTCAAGTGTGGAAACCGCAG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	59.0	56.6	905-988 bp	1.0	69.4
atpB-rbcL_p5	TCTTCAAGTGTGGAAACCGC	AGTCTCTGTTTGTGGTGACAT	50.0	42.9	58.7	57.2	907-990 bp	1.0	67.8