Hotspot

psaA-ycf3

LSC · 42253-42909 · 657 bp

Back to Strumaria

Pi 0.0126

MarkerSeek score 70.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 657
variable_sites: 19
pi: 0.0126
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	CGACCAAGCTGCTGAGTATT	50.0	50.0	57.9	58.0	815-836 bp	1.0	83.0
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	ACCAAGCTGCTGAGTATTGG	50.0	50.0	57.9	57.9	813-834 bp	1.0	82.2
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	742-763 bp	1.0	82.2
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	746-767 bp	1.0	82.0
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	741-762 bp	1.0	81.2