primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	trnG-GCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	9615	9751	209-216	1.000000	70.1
trnG-GCC-trnR-UCU_p2	trnG-GCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	9615	9751	212-219	1.000000	69.8
trnG-GCC-trnR-UCU_p3	trnG-GCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	9615	9751	215-222	1.000000	69.3
trnG-GCC-trnR-UCU_p4	trnG-GCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	9615	9751	218-225	1.000000	68.9
trnG-GCC-trnR-UCU_p5	trnG-GCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	9615	9751	213-220	1.000000	67.5
psaA-ycf3_p1	psaA-ycf3	1	CCCATTCCTCGAAAGACGTT	CGACCAAGCTGCTGAGTATT	20	20	50.0	50.0	57.9	58.0	0.0	1.4	0.110498	42253	42909	815-836	1.000000	83.0
psaA-ycf3_p2	psaA-ycf3	2	CCCATTCCTCGAAAGACGTT	ACCAAGCTGCTGAGTATTGG	20	20	50.0	50.0	57.9	57.9	0.0	12.9	0.214563	42253	42909	813-834	1.000000	82.2
psaA-ycf3_p3	psaA-ycf3	3	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.258580	42253	42909	742-763	1.000000	82.2
psaA-ycf3_p4	psaA-ycf3	4	CCCATTCCTCGAAAGACGTT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.286351	42253	42909	746-767	1.000000	82.0
psaA-ycf3_p5	psaA-ycf3	5	CCCATTCCTCGAAAGACGTT	TGAAGATCACGAGGCGTTTC	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.384749	42253	42909	741-762	1.000000	81.2
atpB-rbcL_p1	atpB-rbcL	1	TCTTCAAGTGTGGAAACCGC	AGTCTCTGTTTGTGGTGACA	20	20	50.0	45.0	58.7	56.6	0.0	0.0	2.066495	55712	56587	907-990	1.000000	71.6
atpB-rbcL_p2	atpB-rbcL	2	CGCAGGACCAGAAGTAGTAG	AGTCTCTGTTTGTGGTGACA	20	20	55.0	45.0	57.1	56.6	0.0	0.0	2.225427	55712	56587	890-973	1.000000	70.4
atpB-rbcL_p3	atpB-rbcL	3	CTTCAAGTGTGGAAACCGCA	AGTCTCTGTTTGTGGTGACA	20	20	50.0	45.0	59.0	56.6	0.0	0.0	2.350494	55712	56587	906-989	1.000000	69.4
atpB-rbcL_p4	atpB-rbcL	4	TTCAAGTGTGGAAACCGCAG	AGTCTCTGTTTGTGGTGACA	20	20	50.0	45.0	59.0	56.6	4.2	0.0	2.350494	55712	56587	905-988	1.000000	69.4
atpB-rbcL_p5	atpB-rbcL	5	TCTTCAAGTGTGGAAACCGC	AGTCTCTGTTTGTGGTGACAT	20	21	50.0	42.9	58.7	57.2	0.0	0.0	2.540580	55712	56587	907-990	1.000000	67.8
rbcL-accD_p1	rbcL-accD	1	CGCTGCTTGTGAAGTATGGA	CAACATCGAATTGAACCGCC	20	20	50.0	50.0	58.3	58.1	0.0	6.9	0.379308	58028	58817	858-922	1.000000	83.8
rbcL-accD_p2	rbcL-accD	2	CGCTGCTTGTGAAGTATGGA	ACATCGAATTGAACCGCCAT	20	20	50.0	45.0	58.3	58.2	0.0	0.0	0.524166	58028	58817	856-920	1.000000	82.6
rbcL-accD_p3	rbcL-accD	3	CGCTGCTTGTGAAGTATGGA	AACATCGAATTGAACCGCCA	20	20	50.0	45.0	58.3	58.5	0.0	0.0	0.745134	58028	58817	857-921	1.000000	80.8
rbcL-accD_p4	rbcL-accD	4	CGCTGCTTGTGAAGTATGGA	ACAACATCGAATTGAACCGC	20	20	50.0	45.0	58.3	57.4	0.0	17.1	0.886333	58028	58817	859-923	1.000000	79.7
rbcL-accD_p5	rbcL-accD	5	CGCTGCTTGTGAAGTATGGA	CATCGAATTGAACCGCCATT	20	20	50.0	45.0	58.3	57.2	0.0	0.0	1.124240	58028	58817	855-919	1.000000	77.8
petA-psbJ_p1	petA-psbJ	1	CGTCCAAGGCCTTTTGTTTT	GGTTCATATTCCGGGTTGGG	20	20	45.0	55.0	57.4	58.3	17.6	0.0	0.907561	64452	65570	1261-1283	1.000000	76.8
petA-psbJ_p2	petA-psbJ	2	CGTCCAAGGCCTTTTGTTTT	CATATTCCGGGTTGGGTTCA	20	20	45.0	50.0	57.4	57.6	17.6	0.0	1.023668	64452	65570	1257-1279	1.000000	75.8
petA-psbJ_p3	petA-psbJ	3	CGTCCAAGGCCTTTTGTTTT	GTTCATATTCCGGGTTGGGT	20	20	45.0	50.0	57.4	57.6	17.6	0.0	1.025371	64452	65570	1260-1282	1.000000	75.8
petA-psbJ_p4	petA-psbJ	4	GATCCATTACGCGTCCAAGG	GGTTCATATTCCGGGTTGGG	20	20	55.0	55.0	58.8	58.3	18.6	0.0	1.094555	64452	65570	1272-1294	1.000000	75.2
petA-psbJ_p5	petA-psbJ	5	CGTCCAAGGCCTTTTGTTTT	CGGTATTCCTGTGATCGGTT	20	20	45.0	50.0	57.4	57.4	17.6	0.0	1.192027	64452	65570	1301-1323	1.000000	74.4
petD-rpoA_p1	petD-rpoA	1	CCGCGAAATACCACGATGTA	ACTCTACAGAAGCATTTCGCA	20	21	50.0	42.9	58.2	57.7	8.2	0.0	1.476417	79193	79396	252-287	1.000000	76.3
petD-rpoA_p2	petD-rpoA	2	GGTACCGCAGTAGCTCTTTG	ACTCTACAGAAGCATTTCGCA	20	21	55.0	42.9	58.4	57.7	0.0	0.0	1.677560	79193	79396	340-375	1.000000	73.7
petD-rpoA_p3	petD-rpoA	3	GTACCGCAGTAGCTCTTTGG	ACTCTACAGAAGCATTTCGCA	20	21	55.0	42.9	58.4	57.7	0.0	0.0	1.677560	79193	79396	339-374	1.000000	73.7
petD-rpoA_p4	petD-rpoA	4	CCGCGAAATACCACGATGTA	CACTCTACAGAAGCATTTCGC	20	21	50.0	47.6	58.2	57.4	8.2	0.0	1.805459	79193	79396	253-288	1.000000	73.7
petD-rpoA_p5	petD-rpoA	5	CCGCAGTAGCTCTTTGGTTA	ACTCTACAGAAGCATTTCGCA	20	21	50.0	42.9	57.6	57.7	0.0	0.0	1.699221	79193	79396	336-371	1.000000	73.6
ycf1_p1	ycf1	1	ACAGGAACAAGAGGGATCCA	CGCGTTTGCCTTTTCTTGAA	20	20	50.0	45.0	58.0	58.2	15.2	0.0	0.210933	112106	112225	1037-1049	0.285714	50.6
ycf1_p2	ycf1	2	ACAGGAACAAGAGGGATCCA	ATTCTCCGCCAAAGCTAACC	20	20	50.0	50.0	58.0	58.2	15.2	0.0	0.293355	112106	112225	752-752	0.285714	49.7
ycf1_p3	ycf1	3	ACAGGAACAAGAGGGATCCA	TGCGCGCGTTTAAAAGAAAA	20	20	50.0	40.0	58.0	58.2	15.2	32.3	0.220964	112106	112225	804-804	0.142857	43.9
ycf1_p4	ycf1	4	ACAGGAACAAGAGGGATCCA	TTGCGCGCGTTTAAAAGAAA	20	20	50.0	40.0	58.0	58.2	15.2	32.3	0.220964	112106	112225	805-805	0.142857	43.9
ycf1_p5	ycf1	5	ACAGGAACAAGAGGGATCCA	CTGAATCAATTCCTTGCGCG	20	20	50.0	50.0	58.0	58.2	15.2	0.0	0.225943	112106	112225	818-818	0.142857	43.9
ycf1-rpl32_p1	ycf1-rpl32	1	ATCTCCCAGACGTCGTAGAG	AATGCTTAGAACCGGCAGAC	20	20	55.0	50.0	58.0	58.3	19.5	0.0	0.304637	116518	117226	1333-2423	0.285714	50.4
ycf1-rpl32_p2	ycf1-rpl32	2	ATGGAATGCCCCCTTGAATC	AATGCTTAGAACCGGCAGAC	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.353532	116518	117226	1744-2834	0.285714	50.0
ycf1-rpl32_p3	ycf1-rpl32	3	GATGGAATGCCCCCTTGAAT	AATGCTTAGAACCGGCAGAC	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.353532	116518	117226	1745-2835	0.285714	50.0
ycf1-rpl32_p4	ycf1-rpl32	4	GGAATGCCCCCTTGAATCAA	AATGCTTAGAACCGGCAGAC	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.396264	116518	117226	1742-2832	0.285714	49.6
ycf1-rpl32_p5	ycf1-rpl32	5	ACCAATAACGGCGGTCATTT	AATGCTTAGAACCGGCAGAC	20	20	45.0	50.0	58.2	58.3	13.2	0.0	0.431954	116518	117226	1095-2185	0.285714	49.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TAGACACGGCACTATGAGCT	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.360309	117401	118108	1189-1212	0.714286	71.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TAGACACGGCACTATGAGCT	TGGCACTTGGCACAGTTATT	20	20	50.0	45.0	58.2	58.0	0.0	0.5	0.242076	117401	118108	1626-1650	0.714286	71.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TAGACACGGCACTATGAGCT	TGGCGAAACCTTGGGTAAAA	20	20	50.0	45.0	58.2	57.9	0.0	0.0	0.304866	117401	118108	1647-1671	0.714286	70.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TAGACACGGCACTATGAGCT	GGGTAAAAATGGCACTTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.329338	117401	118108	1635-1659	0.714286	70.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TAGACACGGCACTATGAGCT	ACTGAAAATAACGACCCGCA	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.408883	117401	118108	1780-1804	0.714286	69.5
psaC-ndhE_p1	psaC-ndhE	1	GGACAAGCGGATTCACATCT	GGAAGCAGCTATTGGGCTAG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.140294	121339	121860	699-789	0.857143	82.3
psaC-ndhE_p2	psaC-ndhE	2	GGACAAGCGGATTCACATCT	GGGTCACTTCACTGGTTTGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.082621	121339	121860	1330-1415	0.857143	81.7
psaC-ndhE_p3	psaC-ndhE	3	GGACAAGCGGATTCACATCT	GTTGGAGATGGGGTCACTTC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.145181	121339	121860	1340-1425	0.857143	81.2
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	GGGTCACTTCACTGGTTTGT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.178653	121339	121860	1256-1341	0.857143	81.1
psaC-ndhE_p5	psaC-ndhE	5	TTCTCGAGTACGGGCTTTTG	GGGTCACTTCACTGGTTTGT	20	20	50.0	50.0	57.9	57.9	13.1	0.0	0.127329	121339	121860	1552-1552	0.142857	44.7
rps15-ycf1_p1	rps15-ycf1	1	ACAAATAATCCAGCAGCCGT	ATCTCCCAGACGTCGTAGAG	20	20	45.0	55.0	57.9	58.0	0.0	19.5	0.172474	127666	128042	782-895	0.857143	84.9
rps15-ycf1_p2	rps15-ycf1	2	CCACCGTGTTACATAAGGCA	ATCTCCCAGACGTCGTAGAG	20	20	50.0	55.0	57.9	58.0	0.0	19.5	0.145773	127666	128042	1189-1303	0.857143	83.7
rps15-ycf1_p3	rps15-ycf1	3	TTTTTCGTAGACCCCCTTGC	ATCTCCCAGACGTCGTAGAG	20	20	50.0	55.0	58.1	58.0	0.0	19.5	0.143458	127666	128042	844-844	0.142857	44.6
rps15-ycf1_p4	rps15-ycf1	4	TTTTTCGTAGACCCCCTTGC	ATGGAATGCCCCCTTGAATC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.192353	127666	128042	1255-1255	0.142857	44.2
rps15-ycf1_p5	rps15-ycf1	5	TTTTTCGTAGACCCCCTTGC	GATGGAATGCCCCCTTGAAT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.192353	127666	128042	1256-1256	0.142857	44.2