Hotspot

ycf1

SSC · 125219-130711 · 5493 bp

Back to Strophanthus

Pi 0.0141

MarkerSeek score 51.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5493
variable_sites: 116
pi: 0.0141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCTTGTGACAAATAGGATAAGTCA	ACACAACTTCTTCTTGAATCGA	37.5	36.4	57.0	56.0	197 bp	1.0	44.1
ycf1_p2	GCTTGTGACAAATAGGATAAGTCA	TCCAGACTCAATACACAACTTCT	37.5	39.1	57.0	57.2	209 bp	1.0	43.8
ycf1_p3	TGCTTGTGACAAATAGGATAAGTC	TCCAGACTCAATACACAACTTCT	37.5	39.1	57.0	57.2	210 bp	1.0	43.8
ycf1_p4	TGCTTGTGACAAATAGGATAAGT	TCCAGACTCAATACACAACTTCT	34.8	39.1	55.9	57.2	210 bp	1.0	43.8
ycf1_p5	ACATATGCTTGTGACAAATAGGA	TCCAGACTCAATACACAACTTCT	34.8	39.1	56.0	57.2	215 bp	1.0	43.7