Hotspot

psaC

SSC · 118665-118910 · 246 bp

Back to Strophanthus

Pi 0.00813

MarkerSeek score 46.10

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 3
pi: 0.00813
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	CACGGGTCTTGTCGGTAAAA	ACTAGGTCGAAAATTTATGACGT	50.0	34.8	58.1	56.0	346-347 bp	1.0	40.7
psaC_p2	CACGGGTCTTGTCGGTAAAA	AGGTCGAAAATTTATGACGT	50.0	35.0	58.1	52.9	343-344 bp	1.0	40.7
psaC_p3	CACGGGTCTTGTCGGTAAAA	AGGTCGAAAATTTATGACGTT	50.0	33.3	58.1	53.9	343-344 bp	1.0	40.7
psaC_p4	CACGGGTCTTGTCGGTAAAA	ACTAGGTCGAAAATTTATGACGTT	50.0	33.3	58.1	56.7	346-347 bp	1.0	40.7
psaC_p5	CACGGGTCTTGTCGGTAAAA	AGGTCGAAAATTTATGACGTTT	50.0	31.8	58.1	54.7	343-344 bp	1.0	40.7