Hotspot

ccsA-ndhD

SSC · 116791-117042 · 252 bp

Back to Strophanthus

Pi 0.0159

MarkerSeek score 66.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 252
variable_sites: 6
pi: 0.0159
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATTCGGCAATTGTAGCGTCT	ATCGGTTGACAAGGTCGAAG	45.0	50.0	58.0	57.9	409-419 bp	1.0	81.9
ccsA-ndhD_p2	ATTCGGCAATTGTAGCGTCT	CGGTTGACAAGGTCGAAGTT	45.0	50.0	58.0	58.4	407-417 bp	1.0	79.1
ccsA-ndhD_p3	ATTCGGCAATTGTAGCGTCT	ACCCGGATTTCGTTCTTTCA	45.0	45.0	58.0	57.4	431-441 bp	1.0	77.9
ccsA-ndhD_p4	ATTCGGCAATTGTAGCGTCT	ACTATCGGTTGACAAGGTCG	45.0	50.0	58.0	57.4	412-422 bp	1.0	77.3
ccsA-ndhD_p5	TTCGGCAATTGTAGCGTCTA	ATCGGTTGACAAGGTCGAAG	45.0	50.0	57.3	57.9	408-418 bp	1.0	76.8