Hotspot

rps4-trnT-UGU

LSC · 47207-47595 · 389 bp

Back to Strophanthus

Pi 0.0209

MarkerSeek score 66.10

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 389
variable_sites: 12
pi: 0.0209
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TCCCGAACGAGATTGGTTTC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	563-577 bp	1.0	80.8
rps4-trnT-UGU_p2	CCCGAACGAGATTGGTTTCT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	562-576 bp	1.0	80.7
rps4-trnT-UGU_p3	TCCCGAACGAGATTGGTTTC	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.9	57.8	531-545 bp	1.0	80.0
rps4-trnT-UGU_p4	GATCATTTCCGGCTCTAGGC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.2	57.9	540-554 bp	1.0	80.0
rps4-trnT-UGU_p5	CCCGAACGAGATTGGTTTCT	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.9	57.8	530-544 bp	1.0	80.0