Hotspot

atpH-atpI

LSC · 13541-14690 · 1150 bp

Back to Strophanthus

Pi 0.0203

MarkerSeek score 65.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1150
variable_sites: 34
pi: 0.0203
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1237-1248 bp	1.0	82.6
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CTGCGGCTTATATCGGTGAA	45.0	50.0	58.0	57.9	1223-1234 bp	1.0	82.5
atpH-atpI_p3	TACCCTCTACAGCTTGACCC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.1	57.9	1320-1331 bp	1.0	81.5
atpH-atpI_p4	GCGATACCCTCTACAGCTTG	TTTTGCAACTTTAGCTGCGG	55.0	45.0	57.9	57.9	1324-1335 bp	1.0	81.5
atpH-atpI_p5	TACCCTCTACAGCTTGACCC	CTGCGGCTTATATCGGTGAA	55.0	50.0	58.1	57.9	1306-1317 bp	1.0	81.4