Hotspot

trnS-GCU-trnG-UCC

LSC · 8456-8996 · 541 bp

Back to Strophanthus

Pi 0.0168

MarkerSeek score 65.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 541
variable_sites: 13
pi: 0.0168
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	TCAAACCGAAAACAAAGACCC	45.0	42.9	58.0	57.2	647-660 bp	1.0	68.7
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	CAAACCGAAAACAAAGACCCT	45.0	42.9	58.0	57.2	646-659 bp	1.0	68.7
trnS-GCU-trnG-UCC_p3	TCAAGTCATCAAGGGAAGCG	TCAAACCGAAAACAAAGACCC	50.0	42.9	57.9	57.2	713-726 bp	1.0	67.9
trnS-GCU-trnG-UCC_p4	TCAAGTCATCAAGGGAAGCG	CAAACCGAAAACAAAGACCCT	50.0	42.9	57.9	57.2	712-725 bp	1.0	67.9
trnS-GCU-trnG-UCC_p5	CATCAAGGGAAGCGGAAAGA	TCAAACCGAAAACAAAGACCC	50.0	42.9	57.9	57.2	707-720 bp	1.0	67.8