Hotspot

trnH-GUG-psbA

LSC · 80-415 · 336 bp

Back to Strophanthus

Pi 0.0242
MarkerSeek score 64.30
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
336
variable_sites
12
pi
0.0242
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 390-418 bp 1.0 84.0
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 393-421 bp 1.0 83.9
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 404-432 bp 1.0 83.8
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 403-431 bp 1.0 83.8
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 405-433 bp 1.0 83.7